Table 4.
Sequences of the forward and reverse primers used to clone European hamster genes of interest.
| Genes | Reference sequences for primers designa | Forward Primer Sequencesb | Reverse Primer Sequencesc | Cricetus cricetus GenBank Accession Numbersd |
|---|---|---|---|---|
| Per1 | NM_001034125.1: Rattus norvegicus period circadian clock 1 (Per1) | TGTGCACCCCTGGAGCCGCAAGG | TTTCTTGGCCCCCACAGGAACTG | MG598318: [Cricetus cricetus] period circadian clock 1 (Per1) mRNA, partial CDS |
| Bmal1 | AB012600: Rattus norvegicus mRNA for BMAL1b | TAAAACGGATATAACCCCTGGGC CTGCCCTCTGGAGAAGGTGGCCC | TCTGGTTCCCCCTGGAATGCCTG ACCCAGCCCCGCATCTGCTTCCA | MG598320: [Cricetus cricetus] BmalI aryl hydrocarbon receptor nuclear translocator like (Arntl) mRNA, partial CDS |
| Rev-Erbα | XM_003498212.2: Cricetulus griseus nuclear receptor subfamily 1, group D, member 2 (Nr1d2) | GCTCTAACTCTGATGCCAACGG | GCTTTTGAGGTTTTCTTGCTCCAG | MG598307: [Cricetus cricetus] Nuclear receptor subfamily 1 group D member 2 (Nr1d2) mRNA, partial CDS |
| Clock | XM_016980269: Cricetulus griseus clock circadian regulator (Clock) | TCAATTGTTGACAGAGATGACAGTAG | TCTATTGTTCCTCGAAGCATGTGAC | MG598315: [Cricetus cricetus] circadian locomotor output cycles protein kaput (Clock) mRNA, partial CDS |
| Per2 | XM_007622995: Cricetulus griseus period circadian clock 2 (Per2) | ACTGTGATGACAATGGGAAGGAGCT | ATGGAGGCAACTTGGTTAGAGATGT | MG598316: [Cricetus cricetus] Period circadian protein 2 (Per2) mRNA, partial CDS |
| MT 1 | U14110.1: Phodopus sungorus melatonin receptor (Mel-1a) | ATGAAGGGCAATGGTAGCACTCTGCTCAATGCC | CCGTATATAATTGCATTGAGGCAGCTG | MG598322: [Cricetus cricetus] Melatonin receptor 1 A (Mtnr1a) mRNA, complete CDS |
| MT 2 | NM_145712.2: Mus musculus melatonin receptor 1B (Mtnr1b) | TTGTTTGTGGTGAGTCTGGTCTTGG | GCCCATAGACAATGACGTTAAGGCAG | MG598323: [Cricetus cricetus] Melatonin receptor 1B (Mtnr1b) mRNA, partial CDS |
| GPR50 | XM_007631612: Cricetulus griseus G protein-coupled receptor 50 (Gpr50) | CCGAACTGGCTGTATCTTGCAG | TCATACAGCCATCTCATCAGAA | MG598317: [Cricetus cricetus] G protein-coupled receptor 50 (Gpr50) mRNA, partial CDS |
| QR2 | XM_007638944.1: Cricetulus griseus NAD(P)H dehydrogenase, quinone 2 (Nqo2) | TGGCAGGTAAGAAAGTGCTCATC/TCACTGGTTCCCTCTCTAATCCTG | TCTTCAGCCGCTTCGCCCATGATGC/TCTTCAGCCGCTTCGCCCATGATGC | KT992792: [Cricetus cricetus] NAD(P)H dehydrogenase quinone 2 mRNA, partial CDS |
| Tubulin | NM_001243978: Cricetulus griseus tubulin, alpha 1 A (Tuba1a) | ACACCTTCTTCAGTGAGACAGGCG | CCCAAAGATGTCAATGCTGCC | MG598321: [Cricetus cricetus] tubulin alpha 1B (Tuba1b) mRNA, partial CDS |
| Actin | NM_001244575: Cricetulus griseus actin beta (Actb) | CCCATTGAACACGGCATTGTC | CGACATCCGCAAAGACCTCTATG | MG598319: [Cricetus cricetus] actin beta (Actb) mRNA, partial CDS |
| SIRT1 | XM_005070811.1: Mesocricetus auratus sirtuin 1 (Sirt1) | GTCATAGGTTAGGTGGTGAATATGCC | CACAGGAACTAGAGGATAAGATGTCGTC | MG598314: [Cricetus cricetus] sirtuin 1 (Sirt1) mRNA, partial CDS |
| TXNIP | XM_003498621.2: Cricetulus griseus thioredoxin interacting protein (Txnip) | CGACTCAGGAGGCAAAGAAAAAC | CAATCACCAGGGGAAGGTCAAG | MG598310: [Cricetus cricetus] Thioredoxin interacting protein (Txnip) mRNA, partial CDS. |
| PPARα | XM_007621010.1: Cricetulus griseus peroxisome proliferator-activated receptor alpha (Ppara) | GAATAAGTGCCAATACTGCCGC | CATACGCTATCAGCATCCCGTC | MG598312: [Cricetus cricetus] Peroxisome proliferator-activated receptor alpha (Ppara) mRNA, partial CDS |
| PGC1α | XM_007620649.1: Cricetulus griseus peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (Ppargc1a) | TTTGATGTGTCGCCTTCTTGC | GGTGTAACGGTAGGTGATGAAACC | MG598311: [Cricetus cricetus] Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (Ppargc1a) mRNA, partial CDS. |
| FGF21 | XM_007638697.1: Cricetulus griseus fibroblast growth factor 21 (Fgf21) | TGGACTGGATGAAATCTGGAGTTG | AAGGTCCCACCATGCTCAGTGG | MG598309: [Cricetus cricetus] Fibroblast growth factor 21 (Fgf21) mRNA, partial CDS |
| Ob-R | XM_007632623.1: Cricetulus griseus leptin receptor (Lepr) | GCCTGTCTTTCCAGAGAATAACCTTC | CGGCACTCACTTTACTCATTGGC | MG598308: [Cricetus cricetus] Leptin receptor (Lepr) mRNA, partial CDS |
| UCP1 | NM_001281332.1: Mesocricetus auratus uncoupling protein 1 (Ucp1), mRNA | TCTACGATACTGTCCAGGAGTACTTC | CAGTCCACCGTCTGCCTCGACT | MG598313: [Cricetus cricetus] Uncoupling protein 1 (UCP1) mRNA, partial CDS |
To obtain the sequences of the unpublished genes of interest, forwardb and reversec primers were designed using published sequencesa. These sequences have been submitted to GenBankd.