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. 2018 Sep 4;4:49. doi: 10.1038/s41421-018-0051-0

Fig. 1. Wnt activation and BMP inhibition are sufficient to maintain Lgr5+ ISCs.

Fig. 1

a Representative bright-field images of small intestinal organoids cultured with EGF, Noggin and R-spondin1 (ENR); EGF and R-spondin1 (ER); Noggin and R-spondin1 (NR); and EGF and Noggin (EN) for 7 days. b, c GFP fluorescence images (b) and FACS analysis of GFP expression (c) of small intestinal organoids cultured with ENR, ER and NR for 7 days. d, e GFP fluorescence images (d) and FACS analysis of GFP expression (e) of small intestinal organoids cultured with NR, CHIR and Noggin (CN) and CHIR and LDN (2ki) for 7 days. f EdU staining of proliferating cells in small intestinal organoids cultured with NR, CN and 2ki for 7 days. Scale bars, 100 μm. **P < 0.01 and *P < 0.05 analyzed by two-way ANOVA test. Error bars, s.d. n = 3 mice in c and n = 4 mice in e. Refer to Supplementary Figure 1 and 2 for detailed analysis