Fig. 2.
Kif11/Eg5 inactivation by STLC reduces spindle chirality, whereas latrunculin A treatment does not. a Horizontal spindle in a live HeLa cell expressing PRC1-GFP with SiR-DNA-labeled chromosomes, treated with STLC (left); arrows connecting starting and ending points of bundles traced upwards, from the same cell (right). Circle denotes spindle axis, and colors show z-distance between starting and ending points, see color bar. b–e Helicity of spindles before and after STLC or DMSO (mock) treatment, as indicated. c Helicity before treatment was different from zero (p = 10−44), but not at 5 and 10 min (p = 0.21 and 0.28). d All helicities were different from zero (p = 10−44, 7 × 10−9, and 4 × 10−9 at 0, 5, and 10 min). f Spindle of a live HeLa cell treated with latrunculin A, legend as in a. g Helicity before and after treatment with latrunculin A. In all panels live HeLa cells expressing PRC1-GFP were used, except in e where live U2OS cells expressing CENP-A-GFP and mCherry-α-tubulin were used. In b–e and g, numbers represent the number of cells (top) and bundles (bottom), from 5 independent experiments in b-e and from 4 independent experiments in g; ***p < 0.001, *0.01 < p < 0.05, n.s., not significant; all p values from a Student's t-test (two-tailed and two-sample unequal-variance) are given in Supplementary Fig. 2a. Images are maximum intensity projections of five central planes. Scale bars, 1 μm; error bars, s.e.m