Fig. 3. Immunohistochemistry for Iba1 and CD68 in the ischemic rat brain. (A) Iba1-immunostaining. Within the 1st day, the microglia underwent morphological changes to an activated amoeboid form in the core and penumbra. In the subacute phase (3~7 days), a mixed population of microglia/macrophages increased in the core and penumbra. (B) Quantitative measurement of Iba1-positive cells in the boxed area of 1 mm2. (C) CD68 immunostaining. Note the absence of CD68-positive cells in the sham group. The boxed areas in the full-size brain images are magnified to the representative image. (D) Quantitative measurement of CD68-positive cells in the boxed area of 1 mm2. Data are presented as means±SEM per mm2 obtained from four animals per group (*p<0.05, ***p<0.001, compared to the value of the sham group; #p<0.05, ##p<0.01, ###p<0.001, compared to the values of the 0 h time point). Scale bar, 20 µm.
