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. 2018 Aug 28;9:2014. doi: 10.3389/fmicb.2018.02014

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Copyright © 2018 Leaden, Silva, Ribeiro, dos Santos, Lorenzetti, Alegria, Schulz, Medeiros, Koide and Marques.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of iron-dependent regulation of imuA expression. Expression was determined by β-galactosidase activity assays of each strain harboring imuA/lacZ transcriptional fusions. Cultures were grown in either M2 (Fe+/DP–), iron-supplemented M2 (with 100 μM FeSO₄, Fe++/DP–), DP-treated M2 for 4 h (with 100 μM DP, Fe+/DP+) or iron-limited M2 (Fe–/DP–) for 4 h. The ΔrecA mutant was grown in the same conditions, except for iron supplemented M2. Bars with asterisks (^∗) are significantly different to wt grown in M2; and (^∗∗) indicates that results from DP-treated M2 and iron-limited M2 are significantly different (P < 0.05) by one-way ANOVA test.