Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype and use or description | Source or reference |
|---|---|---|
| Escherichia coli strain | ||
| DH5α | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ- thi-1 gyrA96 relA1; cloning strain | Invitrogen, Austria |
| Tannerella forsythia strains | ||
| ATCC 43037 | Type strain, wild-type | ATCC; Friedrich et al., 2015 |
| ATCC 43037 ΔTanf_01245 | ΔTanf_01245::ermF; knock-out strain of Tanf_01245 | Tomek et al., 2017 |
| ATCC 43037 ΔTanf_01245+ | ΔTanf_01245::Tanf_01245 cat; reconstituted knock-out strain | Tomek et al., 2017 |
| ATCC 43037 ΔTanf_01250 | ΔTanf_01250::ermF; knock-out strain of Tanf_01250 | This work |
| ATCC 43037 ΔTanf_01255 | ΔTanf_01255::ermF; knock-out strain of Tanf_01255 | This work |
| ATCC 43037 ΔTanf_01260 | ΔTanf_01260::ermF; knock-out strain of Tanf_01260 | This work |
| ATCC 43037 ΔTanf_01260+ | ΔTanf_01260::Tanf_01260 cat; reconstituted knock-out strain | This work |
| ATCC 43037 ΔTanf_01290 | ΔTanf_01290::ermF; knock-out strain of Tanf_01290 | This work |
| ATCC 43037 ΔTanf_01290+ | ΔTanf_01290::Tanf_01290 cat; reconstituted knock-out strain | This work |
| ATCC 43037 ΔTanf_01295 | ΔTanf_01295::ermF; knock-out strain of Tanf_01295 | This work |
| ATCC 43037 ΔTanf_01300 | ΔTanf_01300::ermF; knock-out strain of Tanf_01300 | This work |
| ATCC 43037 ΔTanf_01300+ | ΔTanf_01300::Tanf_01300 cat; reconstituted knock-out strain | This work |
| ATCC 43037 ΔTanf_01305 | ΔTanf_01305::(Perm)-ermF; knock-out strain of Tanf_01305 | This work |
| ATCC 43037 ΔTanf_01305+ | ΔTanf_01305::Tanf_01305 cat; reconstituted knock-out strain | This work |
| Plasmids | ||
| pJET1.2/blunt | Cloning vector; ampR | Thermo Fisher Scientific |
| pJET/ΔTF0955ko | Vector for amplification of the erythromycin resistance gene | Tomek et al., 2014 |
| pEXALV | Vector for amplification of the chloramphenicol resistance gene | Zarschler et al., 2009 |
| pJET1.2/ΔTanf_01250 | Tanf_01250 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01250+ | Cassette for reconstitution of ΔTanf_01250; ampR catR | This work |
| pJET1.2/ΔTanf_01255 | Tanf_01255 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01260 | Tanf_01260 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01260+ | Cassette for reconstitution of ΔTanf_01260; ampR catR | This work |
| pJET1.2/ΔTanf_01290 | Tanf_01290 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01290+ | Cassette for reconstitution of ΔTanf_01290; ampR catR | This work |
| pJET1.2/ΔTanf_01295 | Tanf_01295 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01300 | Tanf_01300 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01300+ | Cassette for reconstitution of ΔTanf_01300; ampR catR | This work |
| pJET1.2/ΔTanf_01305 | Tanf_01305 knock-out cassette; ampR ermFR | This work |
| pJET1.2/ΔTanf_01305+ | Cassette for reconstitution of ΔTanf_01305; ampR catR | This work |