Figure 5.

Phenotype of primary bone-marrow derived DCs (1° BM DCs) and Cas9-Hoxb8 DCs in CM-CSF cultures before LPS activation. Flow cytometric analysis of cells cultured for 8 days in the presence of GM-CSF. Without LPS activation, both (A) 1° BM and (B) Cas9-Hoxb8 cells were subdivided on the basis of CD11c, MHCII and CD11b expression. Histograms show surface expression of the markers indicated on MHCII^hiCD11b^lo (red) and MCHII^intCD11b^hi (blue) myeloid cell subsets. Data are representative for three independent experiments.