3.

(A) Confocal images (100 x) and Western blot analysis of T98 and U87 glioblastoma cell lines stably transfected with shLC3A, shLC3B and shTFEB, showing successful suppression of protein expression (assessed in the supernatant fraction). (B) Growth delay dose/response curves, assessed on the 7 th day after irradiation, in the T98 and U87 cell lines without and with repression of the LC3A, LC3B, LC3A/B and TFEB protein expression, using siRNA. (C) Cell viability of the T98 and U87 cell lines after 24 h exposure to temozolomide (10 μΜ) or cisplatin (10 μΜ), in wild type and transfected cells with siLC3A, siLC3B or siTFEB (*P < 0.05, ** P < 0.01, *** P < 0.001).