Figure 2.

The hemocytes of infected mosquitoes are present at sites of incurrent and excurrent intracardiac retrograde hemolymph flow. (A–C) Intact infected mosquito showing hemocytes (red) and E. coli-GFP (green) around the thoraco-abdominal ostia (A, magnified in B; dotted squares) and the posterior excurrent openings of the heart (A, magnified in C; dotted circles). (D,E) Images of another infected mosquito showing that hemocytes do not always surround the thoraco-abdominal ostia (D; dotted square) or the posterior excurrent openings (E; dotted circle). Nuclei were labeled blue with Hoechst 33342. Scale bars: 100 μm.