Table 1.

Tissue-based next-generation sequencing (NGS) and blood-based cfDNA NGS.

Gene	LN biopsy (NGS) (2/27/2014)	Blood cfDNA (5/28/2014)
APC	Y935fs*1	Y935N†
BRAF	Not detected	G466E†
EGFR	G465R - subclonal	G465R†, G465E†, S464L†
		Amplified (pCN 2.2)
FAM123B	G348fs*29	Not tested
FGFR1	Amplified	Not tested
KRAS	Not detected	G13D†, G12S†, Q61H†
MET	Not detected	Amplified (pCN 2.3)
NF1	Rearrangement int30	Not tested
TP53	R213*	R213*

^†Minor alterations: Defined as alterations with relative variant allele frequency (rVAF) less than 10% of the alteration with the highest VAF. In this case TP53 R213

^*is the alteration with the highest VAF.