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. 2018 Aug 28;9:858. doi: 10.3389/fphar.2018.00858

FIGURE 5.

FIGURE 5

NaHS prevents the loss of TJ and AJ proteins by inhibiting ER stress and autophagy at 1d post-SCI. (A) TM and Rapa were applied to specifically activate ER stress and autophagy, respectively. The rats were randomly divided into five groups: (i) the Sham group; (ii) the SCI model group; (iii) the SCI model with NaHS treatment (SCI + NaHS) group; (iv) the SCI model with NaHS and TM treatment (SCI + NaHS + TM) group; and (v) the SCI model with NaHS and Rapa treatment (SCI + NaHS + Rapa) group. Representative western blots of P120, β-catenin and Occludin from each group. (B–D) Quantification of the expression of P120, β-catenin, and Occludin. The relative band density value was normalized to that of GAPDH. All data are presented as the mean ± SEM, n = 5. ∗∗∗P < 0.001 versus the sham group. #P < 0.05 and ##P < 0.01 versus the indicated group. (E,F) Representative fluorescence images of β-catenin and P120 (Green) in sections from the spinal cord tissue in each group. Scale bar = 50 μm, n = 5.