Figure 5.

Gli3 and miR-7 effects on the proliferation of NPs in the cerebral cortex. (A,B) Cortical deficiency of Gli3 did not have an impact on the proportion of BrdU⁺ cells vs. DAPI⁺ cells. Cortical deficiency of miR-7 decreased the proportion of BrdU⁺ cells vs. DAPI⁺ cells. (C,D) Cortical deficiency of Gli3 suppressed the population of cells expressing RGC marker Pax6⁺/DAPI⁺, which was partially rescued by silencing miR-7. Silencing miR-7 showed no significant change of the proportion of cells expressing RGC marker Pax6⁺/DAPI⁺ at E15.5. (E,F) Losing Gli3 increased the ratio of IP marker Tbr2⁺/DAPI⁺, which was restored by blocking miR-7. Knockdown of miR-7 did not affect the ratio of IP marker Tbr2⁺/DAPI⁺. (G,H) No difference was observed in Tbr1⁺ cells vs. DAPI⁺ cells in EG mice, which was significantly reduced by silencing miR-7. Cortical deficiency of miR-7 decreased the proportion of Tbr1⁺ cells vs. DAPI⁺ cells. The markers BrdU, Pax6, Tbr2, Tbr1 and DAPI stained for proliferative NPs, RGCs, IPs, newborn neurons and all cells, respectively. Values represent mean ± SEM. n > 9. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. One-way ANOVA with post hoc test was used. Scale bar = 100 μm.