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. 2018 Aug 28;9:1209. doi: 10.3389/fphys.2018.01209

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Copyright © 2018 Comesaña, Velasco, Conde-Sieira, Míguez, Soengas and Morais.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of T1R family taste receptors. mRNA levels of T1R1 (A,D), T1R2 (B,E), and T1R3 (C,F) in hypothalamus (A–C) and telencephalon (D–F) of rainbow trout 6 h after intraperitoneal administration of 0.5 mL⋅100 g^-1 body mass of saline solution alone (control) or containing 40 μmol⋅mL^-1 of L-leucine, 40 μmol⋅mL^-1 of L-valine, or 40 μmol⋅mL^-1 of L-proline. Each value is the mean + SEM of n = 6 fish per treatment. Gene expression results are referred to control group and are normalized by EF1α and β-actin expression. Different letters indicate significant differences (P < 0.05) between different groups. P-values are: for (A), = 0.403; for (B), = 0.463; for (C), C vs L = 0.015, C vs V = 0.029, C vs P = 0.386, L vs V = 0.202, L vs P = 0.414, V vs P = 1; for (D), = 0.642; for (E), = 0.346; and for (F), C vs L = 0.508, C vs V = 0.037, C vs P = 0.813, L vs V = 0.043, L vs P = 0.571, V vs P = 0.035.