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. 2018 Aug 28;9:1209. doi: 10.3389/fphys.2018.01209

FIGURE 6.

FIGURE 6

Parameters related to BCAA metabolism. Activity of BCAT (A,D), and mRNA levels of BCKDE2 (B,E), and BCKDK (C,F) in hypothalamus (A–C) and telencephalon (D–F) of rainbow trout 6 h after intraperitoneal administration of 0.5 mL⋅100 g-1 body mass of saline solution alone (control) or containing 40 μmol⋅mL-1 of L-leucine, 40 μmol⋅mL-1 of L-valine, or 40 μmol⋅mL-1 of L-proline. Each value is the mean + SEM of n = 10 (enzymatic activity) or n = 6 (mRNA levels) fish per treatment. Gene expression results are referred to control group and are normalized by EF1α and β-actin expression. Different letters indicate significant differences (P < 0.05) between different groups. P-values are: for (A), C vs L = 0.415, C vs V = 0.255, C vs P = 0.432, L vs V = 0.488, L vs P = 0.033, V vs P = 0.661; for (B), C vs L = 0.029, C vs V = 0.750, C vs P = 0.167, L vs V = 0.078, L vs P = 0.166, V vs P = 0.442; for (C), = 0.416; for (D), = 0.718; for (E), C vs L = 0.045, C vs V = 0.026, C vs P = 0.626, L vs V = 0.269, L vs P = 0.792, V vs P = 0.199; and for (F), C vs L = 0.839, C vs V = 0.028, C vs P = 0.451, L vs V = 0.036, L vs P = 0.489, V vs P = 0.026.