Table 1.
Gene primers | Sequence |
---|---|
MBP | F: 5'-GTAGTAAGCCACTCCTTGACTG-3' R: 5'-GCAGAGAGGACTGTTGACAT-3' |
Olig2 | F: 5'-CGCAGCGAGCACCTCAAATCTAA-3' R: 5'-CCCAGGGATGATCTAAGCTCTCGAA-3' |
PDGFRα | F: 5'- GTG GGA CAT TCA TTG CGG A-3' Rev: 5' AAG CTG GCA GAG GAT TAG G-3' |
GFAP | F: 5'- CCGACAGCAGGTCCATGTG-3' Rev: 5'-GTTGCTGGACGCCATTGC-3' |
GAPDH | F: 5'- GAAATCCCATCACCATCTTCCAGG-3' Rev: 5'-GAGCCCCAGCCTTCTCCATG-3' |
MBP: Myelin basic protein; Olig2: Oligodendrocyte transcription factor; PDGFRα: Platelet-derived growth factor receptor α; GFAP: Glial fibrillary acidic protein; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase