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Ghrelin affects pre-autonomic PVN neurons. Ghrelin depolarizes most pre-autonomic PVN neurons (A). Neurons were defined as pre-autonomic due to the presence of low-threshold spikes (blue arrow) after a hyperpolarizing pulse (B), a representative trace of a depolarization is shown (C). Background indicates 10 nM ghrelin application to the bath, dashed line represents mean baseline membrane potential.
