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. 2018 Aug 24;12:275. doi: 10.3389/fncel.2018.00275

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Copyright © 2018 dos-Santos, Grover, Reis, Ferguson and Mecawi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative recordings and RT-PCRs. The figure shows representative traces for ghrelin responsive neurons and the insets show their respective amplification plots for β-Actin, AVP, OT, CRH, and TRH mRNA. Note, a neuron that depolarizes in response to ghrelin and expresses both AVP and OT mRNA, with the predominance of AVP (A); hyperpolarizations in CRH expressing neurons, either with AVP-CRH coexpression (B) or with CRH only (C); and a hyperpolarization in a TRH neuron (D). Blue background indicates 10 nM ghrelin application; red dashed line represents mean membrane potential; vertical scale bar represents 10 mV; and horizontal scale bar represents 60 s.