FIGURE 2.

αβ CD8⁺ innate-like T cells have memory-like properties. (A) Purified αβ TCRβ^hi CD8⁺ SP thymocytes from Elk4^+/−and Elk4^−/− thymus were activated with PDBu and ionomycin and stained for intracellular IFN-γ 5 h later. Three animals per genotype; error bars represent SEM. Statistical significance: *p < 0.05 (unpaired t test). (B) Proliferation of CFSE-labeled FACS-sorted αβ CD8⁺ SP WT and Elk4^−/− thymocytes stimulated by CD3/CD28 for 48 h. The percentage of divided cells is shown. Two independent experiments were performed. (C) Proliferation of CFSE-labeled FACS-sorted F5 Rag2^−/− and F5 Elk4^−/−Rag2^−/− CD8⁺ thymocytes 48 h following stimulation with NP68 peptide and irradiated splenocytes from C57BL6 (APCs). Two independent experiments were performed.