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. 2018 Jul 24;19(8):2161. doi: 10.3390/ijms19082161

Figure 2.

Figure 2

Figure 2

Expression of the human homologues in ASCs. Alignment of mouse and human amino acid sequences for (A) PLPP5, (B) ITM2C, and (C) CLPTM1L. Symbols indicate conserved (I), highly similar (:), and similar (.) residues. (D) RNAseq showing the expression of PLPP5, CLPTM1L, ITM2C and three established clinical candidates SLC3A2, SDC1 and SLAMF7 in purified human B cell and ASC populations. Data are the mean reads per kilobase per million reads (RPKM) ± SD from 3–5 donors each. Cord naïve B cell (CD19+ CD20+ HLA-DR+ IgG−), blood naïve B cell (CD19+ IgD+ CD27−), tonsil naïve B cell (CD19+ CD38− CD27−), tonsil germinal center (GCB) B cell (CD19+ CD38+ CD27+), blood memory B cell (CD19+ IgD− CD27+ CD38−/low), tonsil plasma cell/plasmablast (PC, CD19+ CD27++ CD38++), bone marrow plasma cell (BM PC, CD138++ CD38+). (E) RNAseq showing the expression of PLPP5, CLPTM1L and ITM2C in human immune cell populations. Data obtained from the BLUEPRINT consortium (www.blueprint-epigenome.eu). Data are the mean fragments per kilobase per million reads (FPKM) ± SD. Blood naïve B cell (n = 5, CD19+ CD20+ CD23+ CD38low), tonsil GCB cell (n = 3, CD19+ CD20+ CD38medium), tonsil PC (n = 21, CD20medium CD38++), blood eosinophil (n = 2, CD66+ CD16−), blood erythroblast (n = 8, CD36+ CD71+ CD235a+), blood neutrophil (n = 16, CD66b+ CD16+), cultured macrophages (n = 18) and conventional dendritic cell cDC (n = 3), blood CD4+ T cell (n = 10, CD3+ CD4+ CD45RA+), blood CD8+ T cell (n = 2, CD3+ CD8+ CD45RA+), blood natural killer (NK) cell (n = 4, CD3− CD56+ CD16dim).