Figure 2.

Protein expression of cell-specific markers in CTB and STB cultures. Protein expression of cytokeratin (CK) 7 and vimentin (vim), specific markers for epithelial cells and mesenchymal cells, respectively. (A–D): Representative protein expression patterns of CK7 and vim in isolated trophoblasts after 24 h (undifferentiated trophoblasts; CTB, panel (A) and (C)) and 72 h (differentiated trophoblasts; STB, panel (B) and (D)) of cultivation was assessed by flow cytometry. Specific staining of CK7 and vimentin is depicted in red; the isotype control staining is shown in blue. The images are shown as overlays on isotype control. The black lines on panels A and B indicate the positive (CK-7) and negative (isotype control) expression patterns. Data were evaluated by using FlowJo data analysis software.