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. 2018 Aug 9;19(8):2334. doi: 10.3390/ijms19082334

Figure 3.

Figure 3

Expression of syncytial markers. (A,B) The mRNA abundance and protein secretion of human chorionic gonadotropin (hCG) in primary undifferentiated (CTB) and differentiated (STB) trophoblast cells. The gene expression of hCG was assessed by real-time RT-PCR and normalized to the reference gene as described in Materials and Methods [36]. Primary trophoblasts were isolated from healthy term placentas as described in Materials and Methods. Data are shown as mean ± SD of 12 independent experiments measured at least in duplicates. The protein secretion of hCG in CTB and STB was measured by using a human hCG ELISA kit following the manufacturer’s instructions. Data are shown as mean ± SD of at least three independent experiments; and (C,D) The mRNA abundance of human placental lactogen (hPL) and endoglin in CTB and STB was evaluated as described above for hCG. Data are shown as mean ± SD of three independent experiments measured at least in duplicates.