Table 3.
Primer sequences for RT-PCR.
| Gene | Sequence Number | Sequence Position | Product Length (bp) | Annealing Temperature (°C) | Sequence (5′to3′) |
|---|---|---|---|---|---|
| β-actin | NM_205518.1 | 625–818 | 194 | 57 | F: GTGCGTGACATCAAGGAGAAGC |
| R: CCACAGGACTCCATACCCAAGA | |||||
| ATP5A1 | NM_204286.1 | 1207–1364 | 158 | 57 | F: GGTATCCGTCCAGCCATCAA |
| R: GCATCCAAATCAGACCCAAACT | |||||
| ATP5B | NM_001031391.2 | 482–637 | 156 | 57 | F: GCCCCATCACAACGAAACAG |
| R: CGCCTCCAAACAAACCAATC | |||||
| ATP5C1 | NM_001278096.1 | 272–411 | 140 | 57 | F: ATTAAGGCACCCGAGGACAA |
| R: ACTTCCTTCCCTGCATTGGA | |||||
| ATP5F1 | XM_417993.4 | 437–644 | 208 | 57 | F: CATTGGAGACTGCCATTGAGG |
| R: TGATCTTGCTCTTTCTGACGCTT | |||||
| ATP5G1 | XM_001233602.3 | 287–536 | 250 | 57 | F: CAGGAGCAGGTATTGGGACA |
| R: TTGTCAGTCTGGAACGCTCT | |||||
| ATP5G3 | NM_001277855.1 | 141–288 | 148 | 57 | F: CCAAAACGCTGTCTCCCAAC |
| R: ACCGAAGACCGTTCCAATACC | |||||
| ATP5H | XM_001232598.3 | 332–551 | 220 | 57 | F: CTGAAGGTCCCTGAACCAGT |
| R: ACTTCTCCCTGTCCAGTCTG | |||||
| ATP5I | NM_001097534.2 | 74–240 | 167 | 57 | F: TCTCGCCCCTCATCAAGTTC |
| R: TGCCAGTTCCTTTGCAATCC | |||||
| ATP5J | XM_004938370.1 | 58–197 | 140 | 58 | F: CACTTGCGGAGAAACATCGGT |
| R: CCTACATCAACAGGTCCTCCAGC | |||||
| ATP5J2 | NM_001257200.1 | 170–263 | 94 | 57 | F: GCCTCGGTGGTATCAGTATGGT |
| R: TACTTCCTGCGGCGGTCAT | |||||
| ATP5L | XM_015298211 | 250–377 | 128 | 57 | F: CCATGGTCAGGAGCTTTCAG |
| R: GCCTCGTTTGCCTATGATCTC | |||||
| ATP5S | NM_001277562.1 | 46–279 | 234 | 57 | F: TCCCCTTCCCCTTTCTTTCC |
| R: CATAGCCTTGATAGCGCACC | |||||
| GH | NM_204359.2 | 104–284 | 181 | 57 | F: TGTTTGCCAACGCTGTGCT |
| R: TTCTGCTGGGCGTCATCCT | |||||
| GHR | NM_001001293.1 | 1070–1299 | 230 | 57 | F: GTCACACAGTTGCTTGGGAG |
| R: TATGCGGCTGTTGGGTATCT | |||||
| IGF1 | NM_001004384.2 | 188–316 | 129 | 58 | F: AGTTCGTATGTGGAGACAGAGGC |
| R: CCAGCCTCCTCAGGTCACAAC | |||||
| IGF1R | NM_205032.1 | 2961–3114 | 154 | 57 | F: TTGTGCTCCCCATTGCTTTC |
| R: GGAACGTACACATCCGAAGC | |||||
| IGFBP2 | NM_205359.1 | 582–793 | 212 | 57 | F: TCACAACCACGAGGACTCAAAG |
| R: GCTGCCCATTCACCGACAT | |||||
| POU1F1 | NM_204319.1 | 560–754 | 195 | 57 | F: ATGTTGGCGAAGCACTGGC |
| R: GCTTCCTCTTCCGCTCATTCA | |||||
| AMPKα2 | NM_001039605.1 | 726–943 | 218 | 57 | F: GGAGGCGTGTTTTACATCCC |
| R: AACTTCTCACAGACCTCCCG | |||||
| AMPKβ2 | NM_001044662.1 | 435–661 | 227 | 57 | F: CCAGTGTTTTCAGCTCCCAC |
| R: GAGGTCCAGGATAGCGACAA | |||||
| AMPKγ3 | NM_001031258.2 | 183–320 | 138 | 57 | F: GCTGGAACCCGACAACAATT |
| R: GCCTTCTTGATCTCCAGGGT | |||||
| mTOR | XM_417614.4 | 119–309 | 191 | 57 | F: TGAAGGGGTCAAGGCAATCC |
| R: GGCGAGCAGTGGTTGTGGAT | |||||
| PRDX1 | NM_001271932.1 | 358–545 | 188 | 56 | F: ACAAGGTGGTTTGGGCACTA |
| R: TCTCATCAACAGAACGGCCA | |||||
| PRDX3 | XM_426543.5 | 414–551 | 138 | 56 | F: TTTCACCTTTGTGTGCCCCA |
| R: TTGCGCGGGGTATTTATCCA | |||||
| PRDX4 | XM_001233999.3 | 595–733 | 139 | 56 | F: TGCACTTAGGGGCCTTTTCA |
| R: TTCTCCATGCTTGTCCGTGT | |||||
| PRDX6 | NM_001039329.2 | 189–340 | 152 | 58 | F: TGAGTTCAGCAAACGCAACG |
| R: GCTCTCGGTCCTTATCAGCG | |||||
| TG | XM_015283114.1 | 2812–2966 | 155 | 57 | F: GCAGCTTTCCAAACCTTCAG |
| R: GGCTCCAGCACAGAGAAAAC | |||||
| TPO | XM_015284944.1 | 1872–2075 | 204 | 58 | F: TGACGCTCAAAAGCATGAAC |
| R: TGCTTTGGTGTTCCACACAT | |||||
| THRA | NM_205313.1 | 355–636 | 282 | 57 | F: AAGCGCAAAAGAAAGAGCAG |
| R: CACGGAGATGCACTTCTTGA | |||||
| THRB | NM_205447.2 | 976–1150 | 175 | 58 | F: TTTCCTCCTGGCATTTGAAC |
| R: CAGGAACAATGGAGGGAAGA |
ATP5B, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATP5C1, ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; ATP5F1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1; ATP5G1, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1; ATP5G3, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3; ATP5H, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit D; ATP5I, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; ATP5J, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6; ATP5J2, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2; ATP5L, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G; ATP5S, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit S; IGFBP2, insulin-like growth factor binding protein 2; POU1F1, POU class 1 homeobox 1; AMPKβ2, adenosine monophosphate-activated protein kinase non-catalytic subunit beta 2; AMPKγ3, adenosine monophosphate-activated protein kinase non-catalytic subunit gamma 3; PRDX, peroxiredoxin; TPO, thyroid peroxidase; THRB, thyroid hormone receptor beta.