Table 4.

Primer sequences for bisulfite-sequencing PCR.

Gene	Chromosome Location	Sequence Position	Product Length (bp)	Annealing Temperature (°C)	Sequence (5′to3′)	Expected No. of CpGs
ATP 5A1	Z	1082–1292	211	55	F: GAGGTTTTTTGATTGTTTTGTTTGT	4
					R: CCTACCACCTATTTCATTACCCTAAT
GH	27	458–611	154	53	F: AAGAAGGGATTTAAGTTTTGATGAG	10
					R: TCCTTCTTAAAACAAAACAACAAAC
GHR	Z	436–768	333	53	F: GTTAAATTGGGATTTATGGGGATAT	6
					R: CAACAACTAAAAACCAAAAAAACTC
IGF1	1	413–661	249	53	F: TTGAGTTGGTTGATGTTTTTTAGTT	5
					R: TCAAATACACTTCCTTTTATACTTTTAACA
IGF1R	10	308–554	247	52	F: GTTGAGGGTTTTTTTTAGATTTTGTT	12
					R: TTATATTCCTCAAATTATAAAACCC
AMPKα2	8	233–463	231	52	F: AGAAATTTAAAATTTGAAATTTTTT	10
					R: AAATCCCTATAAACAACCATATATC
mTOR	21	1690–1890	201	52	F: ATATTTAGGATGGGTTGTTGAAAAT	4
					R: AAATCAAAAAATACCCTTCAAACTC
TG	2	2943–3144	202	53	F: TATTGTTTTTTTTGTGTTGGAGTTT	12
					R: TATATATCCCCCATCAATATCACAC
THRA	27	145–385	241	51	F: GGGATGTTGTAGGGAGTTTAGTATT	19
					R: CCAAACATTAACTACTCTTTCTTTTAC