Figure 5.

Inhibition of p38 MAPK by SB203580 increases survivin expression in primary EOC cells. Primary EOC cells were left untreated or treated with 200 μM carboplatin in the presence of SB203580 (10 μM) or an equal volume of DMSO (the vehicle control) for 48 h. (A) Phosphorylation of p38 MAPK and MAPKAPK2 (a direct substrate target of p38 MAPK), as well as survivin were examined by Western blotting. β-actin was used as the loading control. (B) The Western blotting results of survivin were quantified using Odyssey imaging software. The density of the survivin bands was normalized to that of β-actin. The density of the bands in the DMSO/UT cells was designated as 1. The relative level (fold change) of survivin was shown as mean ± SE of three to six independent experiments. * Significantly different (p < 0.05).