Table 4.
Relative gene expression in HEK-pNF-κB-d2EGFP/Neo L2 (HEK wt) and HEK shRNA RelA (RelA k.d.) cells after irradiation * using the RT2 Profiler PCR array **. The complete data sets are shown in Table A5 and Table A6.
| Radiation Quality | X-rays | 48Ti ions | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose (Gy) | 0.5 | 4.0 | 8.0 | 0.5 | 4.0 | |||||
| Gene Symbol/Cell Line | HEK wt | RelA k.d. | HEK wt | RelA k.d. | HEK wt | RelA k.d. | HEK wt | RelA k.d. | HEK wt | RelA k.d. |
| CCL5 | −1.42 ± 0.24 | 1.55 ± 0.68 | 3.68 ± 1.53 | 2.07 ± 0.90 | 3.34 ± 1.40 | −1.47 ± 0.30 | −1.97 ± 0.17 | −1.89 ± 0.23 | −1.56 ± 0.22 | 1.22 ± 0.53 |
| CD83 | −1.01 ± 0.03 | 1.12 ± 0.06 | 2.47 ± 0.08 | 1.67 ± 0.08 | 3.75 ± 0.18 | 2.27 ± 0.11 | 1.25 ± 0.04 | 1.09 ± 0.05 | 4.37 ± 0.14 | 1.42 ± 0.07 |
| CXCL1 | 1.04 ± 0.20 | 1.06 ± 0.09 | 3.57 ± 0.56 | 1.52 ± 0.12 | 6.96 ± 1.01 | 2.02 ± 0.16 | 1.01 ± 0.19 | −1.48 ± 0.05 | 7.55 ± 1.44 | 1.21 ± 0.10 |
| CXCL2 | −1.35 ± 0.13 | −1.04 ± 0.19 | 2.33 ± 0.33 | 1.55 ± 0.30 | 3.01 ± 0.40 | 1.29 ± 0.25 | 1.30 ± 0.22 | 1.11 ± 0.21 | 3.48 ± 0.59 | 1.00 ± 0.19 |
| CXCL10 | −1.10 ± 0.16 | 1.05 ± 0.08 | 4.68 ± 0.60 | 2.23 ± 0.16 | 14.58± 2.57 | 2.04 ± 0.15 | −1.27 ± 0.14 | −1.30 ± 0.06 | 6.70 ± 1.20 | −1.15 ± 0.06 |
| CXCL8/IL8 | −1.73 ± 0.19 | 1.20 ± 0.19 | 2.42 ± 0.66 | 1.38 ± 0.22 | 3.74 ± 0.86 | 1.01 ± 0.16 | 1.38 ± 0.44 | −1.26 ± 0.13 | 6.10 ± 1.96 | 1.44 ± 0.23 |
| NFKB2 | 1.01 ±0.12 | 1.09 ± 0.18 | 2.93 ± 0.33 | 1.48 ± 0.24 | 4.07 ± 0.45 | 1.62 ± 0.26 | −1.25 ± 0.09 | −1.02 ± 0.16 | 2.52 ± 0.29 | 1.04 ± 0.17 |
| NFKBIA | −1.07 ± 0.08 | −1.13 ± 0.10 | 2.65 ± 0.34 | 1.47 ± 0.17 | 4.26 ± 0.40 | 1.69 ± 0.19 | −1.11 ± 0.08 | 1.05 ± 0.12 | 3.55 ± 0.31 | 1.47 ± 0.17 |
| TNF | 1.01 ± 0.11 | 1.08 ± 0.05 | 5.10 ± 0.99 | 1.17 ± 0.06 | 12.90 ± 1.72 | 1.15 ± 0.05 | 1.08 ± 0.12 | −1.26 ± 0.04 | 6.72 ± 0.76 | −1.12 ± 0.04 |
| TNFRSF1B | −1.81 ± 0.16 | −2.15 ± 0.26 | 1.02 ± 0.22 | 4.10 ± 2.28 | 1.11 ± 0.22 | 2.39 ± 1.33 | 1.07 ± 0.30 | 2.88 ± 1.60 | −2.04 ± 0.14 | −2.65 ± 0.21 |
| VCAM1 | −1.27 ± 0.20 | 1.08 ± 0.05 | 4.24 ± 1.56 | 1.17 ± 0.06 | 2.94 ± 0.87 | 1.15 ± 0.05 | −1.77 ± 0.15 | −1.26 ± 0.04 | −1.10 ± 0.24 | −1.12 ± 0.04 |
* Exposure to X-rays or 48Ti-ions (1000 MeV/n, LET 108 keV/µm). ** The table shows all genes from the NF-κB signaling array, that are more than threefold up- or down-regulated (in bold) in irradiated cells compared to mock-irradiated cells. The mean of the fold up- or downregulation was calculated from up to two independent experiments with untreated cells. Prior to investigating gene expression with RT2 ProfilerTM PCR arrays, a proper RNA and cDNA quality was confirmed. ACTB, B2M, GAPDH, HPRT1 and RPLP0 were used as reference genes to normalize the results.