Table 1.

Hyaluronic acid (HA), chitosan (CS), and pentasodium tripolyphosphate (TPP) solution concentrations (input variables) and HA/CS-TPP nanoparticles (NPs) sizes and ζ potentials (output variables) used in the design of experiment (DoE) approach. SD—standard deviation; PDI—polydispersity index.

DoE Batch #	HA Solution (mg/mL) (Level)	CS Solution (mg/mL) (Level)	TPP Solution (mg/mL) (Level)	Size ± SD (nm)	PDI ± SD	ζ Potential ± SD (mV)
A	1 (−1)	0.3 (−1)	0.25 (−1)	385.2 ± 90.7	0.240 ± 0.096	−1.1 ± 0.3
B	1 (−1)	0.5 (+1)	0.5 (+1)	181.9 ± 65.6	0.171 ± 0.040	−30.9 ± 2.5
C	1 (−1)	0.5 (+1)	0.25 (−1)	195.4 ± 75.2	0.421 ± 0.163	−1.5 ± 0.8
D	1 (−1)	0.3 (−1)	0.5 (+1)	210.8 ± 94.9	0.202 ± 0.086	−10.8 ± 2.8
E	2 (+1)	0.5 (+1)	0.5 (+1)	242.5 ± 197.9	0.334 ± 0.103	−38.5 ± 5.2
F	2 (+1)	0.3 (−1)	0.5 (+1)	320.9 ± 322.4	0.382 ± 0.087	−40.5 ± 8.3
G	2 (+1)	0.3 (−1)	0.25 (−1)	630.5 ± 343.4	0.275 ± 0.069	−25.5 ± 4.6
H	2 (+1)	0.5 (+1)	0.25 (−1)	310.8 ± 162.2	0.272 ±0.023	−18.7 ± 3.9
I	1.5 (0)	0.4 (0)	0.375 (0)	230.3 ± 144.6	0.393 ± 0.042	−13.5 ± 1.6
L	1.5 (0)	0.4 (0)	0.375 (0)	223.1 ± 114.7	0.264 ± 0.062	−12.8 ± 3.2