Figure 1.

Modulation of transcript levels of nicotinic acetylcholine receptors and genes involved in glucose uptake in (a) fragmented and (b) intact adult S. japonicum and (c) eggs after treatment with SjAChE dsRNAs. mRNA levels of these genes, relative to the house-keeping gene PSMD4, were analyzed by qRT-PCR. Data are representative of the mean ± SEM of two separate experiments. p values were calculated using one-way ANOVA to compare the gene fold changes between each RNAi group and the luciferase RNAi control group. * = p value ≤ 0.05, ** = p value ≤ 0.001, *** = p value ≤ 0.0001; p value comparing the fold changes between the targeted gene and PSMD4.