Figure 2.

Effect of knockdown of SjAChE in intact adult S. japonicum worms treated with dsRNA for 4 days determined by Western blotting. (a) Anti-actin antibody (upper) and anti-SjAChE antibody (lower) were used to probe a protein extract of intact adult S. japonicum 4 days after treatment with SjAChE dsRNA (Lane 1); dsLUC (Lane 2); and without dsRNA (Lane 3); (b) signal intensities of bands recognised by anti-SjAChE antibody in panel a (bottom) were measured using the Odyssey Classic Infrared Imager with a scan intensity setting of 5 and a sensitivity of 5. Error bars represent the standard error of the mean (SEM). This experiment was performed twice. LUC, luciferase control group; WT, wild type unsuppressed control worms cultured in the same media; ns, no significant difference. * = p value ≤ 0.05.