Figure 1.

E2, BPA, and OP result in loss of implantation sites. Pregnant mice at gestation day 5.5 (GD 5.5) were sacrificed 48 h after final injection. Implantation sites in uteri were detected by application of Chicago Sky Blue 5 min before sacrifice. All implantation sites in the control group were detected as distinct blue bands. The number of implantation sites was significantly low in the OP group and there were no sites in the ICI and E2 groups. n = 3 mice per group. Statistical significance was determined by one-way ANOVA with the Bonferroni correction test. ** p < 0.01 vs. VE group. VE: vehicle; BPA: bisphenol A; OP: 4-tert-octylphenol; E2: 17β-Estradiol.