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. 2018 Aug 18;19(8):2437. doi: 10.3390/ijms19082437

Figure 2.

Figure 2

Multiple alignment of selected K+ channel pore domains and effect of TEA on TASK-3 and Kv2.1 mutant channels expressed in HEK-293 cells. (A) Amino acid sequence alignment of KcsA, Kv2.1 and TASK-3 pore domains. Gaps are indicated by dashes, letters with gray background are the residues implicated in the TEA binding site (Y82, Y380, A99, A100 and Y205, respectively). The selectivity filter signatures are boxed and the numbers are indicated. The PD1 and PD2 signify pore domains 1 and 2 of TASK-3 channels, respectively. (B) Dose-response curve of TEA on A99Y (red triangle) and A100Y (red square) mutants. The block was analyzed at the end of the test pulse at +80 mV. Results are shown as means ± SEM. The black lines were taken from the fits in Figure 1C and correspond to the TEA inhibition curves for TASK-3 WT and Kv2.1, respectively.