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. 2018 Aug 1;19(8):2258. doi: 10.3390/ijms19082258

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Variation in the reference genes expression using distribution of cycle threshold (Cq) values in line charts. (A) Different tissues in adult shrimps. (B) Five different ovary development stages, O1–5: Stage I, Stage II, Stage III, Stage IV, and Stage V of ovary. (C) Different embryo development stages, US—unfertilized egg stage; CS—cleavage stage; BS—blastula stage; GS—gastrul stage; NS—nauplius stage; ZS—zoea stage; L1—one day after larvae hatched. (D) Different times after hypoxia stress in gill. (E) Different times after infection in hepatopancreas.