Table 1.
Primer pairs for qPCR of candidate reference genes. EIF—eukaryotic translation initiation factor 5A; 18S—18S ribosomal RNA; EF-1α—elongation factor-1α; GAPDH—glyceraldehyde-3-phosphate dehydrogenase; TUB—α-tubulin; β-act—β-actin; RPL18—Ribosomal protein L18.
| Gene | Primer Sequence (5′–3′) Forward/Reverse | Length (bp) | PCR Efficiency (%) |
Correlation Coefficient (R2) |
|---|---|---|---|---|
| EIF | CATGGATGTACCTGTGGTGAAAC CTGTCAGCAGAAGGTCCTCATTA |
179 | 94.4 | 0.994 |
| 18S | GTTGGATGTTGCTGTTGAGAGAG CTGGGCATCATTCTCTGGGTAAA |
250 | 96.8 | 0.997 |
| EF-1α | CAAGGATCTGAAACGTGGCTTC GTACGTCTGTCGATCTTGGTCAG |
198 | 93.7 | 0.999 |
| GAPDH | GTCGGTAAGGTCATTCCAGAGC CGAAAGTTTTGCTGAGCTGGAT |
274 | 95.5 | 0.991 |
| TUB | AGGAATGGAAAATCAGGAAGCCC GTTTGTCGATCTGGAACCCTCT |
224 | 97.0 | 0.996 |
| β-act | CACGAGACCACCTACAATTCCA ATAGAGAAGCCAAGATAGAACCGC |
226 | 99.4 | 0.991 |
| RPL18 | CTTTTTGTACCCACAGCTTGACC CACTTTTGATGTATTGGCCCGTC |
202 | 102.7 | 0.997 |