Table 2.
Detailed distribution of cycle threshold (Cq) value information.
| Experimental Conditions | Statistics | Reference Gene | ||||||
|---|---|---|---|---|---|---|---|---|
| EIF | 18S | EF - 1 α | GAPDH | TUB | β-act | RPL18 | ||
| Hypoxia stress | Group | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Mean | 21.86 | 28.19 | 24.20 | 21.19 | 23.80 | 24.35 | 21.33 | |
| Min Cq | 20.64 | 26.08 | 22.50 | 20.02 | 23.13 | 23.01 | 19.03 | |
| Max Cq | 22.33 | 28.98 | 26.42 | 22.66 | 24.56 | 25.66 | 22.43 | |
| WSSV infection | Group | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| Mean | 24.82 | 32.02 | 34.89 | 26.78 | 30.44 | 26.47 | 24.40 | |
| Min Cq | 24.12 | 30.97 | 34.22 | 26.26 | 29.98 | 25.72 | 23.76 | |
| Max Cq | 25.30 | 33.28 | 36.04 | 27.44 | 31.34 | 27.64 | 24.96 | |
| Different adult tissues | Group | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
| Mean | 21.65 | 27.81 | 25.05 | 22.55 | 25.86 | 20.82 | 21.83 | |
| Min Cq | 20.47 | 26.09 | 18.57 | 17.90 | 22.61 | 18.64 | 20.56 | |
| Max Cq | 23.59 | 30.26 | 32.62 | 26.74 | 29.17 | 23.36 | 23.88 | |
| Different ovarian stages | Group | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Mean | 21.29 | 25.42 | 33.34 | 23.61 | 23.81 | 19.28 | 22.18 | |
| Min Cq | 20.24 | 23.77 | 31.56 | 20.71 | 21.06 | 17.75 | 20.59 | |
| Max Cq | 22.66 | 27.62 | 35.32 | 25.40 | 25.11 | 21.19 | 24.40 | |
| Different embryo stages | Group | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
| Mean | 21.23 | 27.51 | 30.90 | 25.13 | 25.25 | 21.71 | 22.63 | |
| Min Cq | 18.58 | 25.37 | 21.12 | 21.25 | 22.53 | 18.26 | 18.95 | |
| Max Cq | 23.77 | 32.10 | 37.48 | 30.47 | 30.98 | 25.62 | 27.97 | |
‘Group’ in this table represents how many states or tissues the genes are studied in. Each means five time points during hypoxia stress, six time points during WSSV infection, seven different organizations in adult tissues study, five different ovarian stages, and seven different embryo stage. WSSV—white spot syndrome virus.