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Ranking of reference genes of RNAi by different methods.
| Experimental Conditions | Method | Rank | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | ||
| RNAi (Androgenic glands) | Comparative ∆Ct | β-act | RPL18 | EIF | TUB | 18S | GAPDH | EF-1α |
| BestKeeper | RPL18 | TUB | β-act | EIF | 18S | GAPDH | EF-1α | |
| NormFinder | β-act | EIF | RPL18 | TUB | GAPDH | 18S | EF-1α | |
| GeNorm | β-act/RPL18 | TUB | EIF | 18S | GAPDH | EF-1α | ||
| Recommended comprehensive ranking | β-act | RPL18 | TUB | EIF | 18S | GAPDH | EF-1α | |
