Table A2.

Ranking of reference genes under different conditions by different methods.

Experimental Conditions	Method	Rank
		1	2	3	4	5	6	7
Different adult tissues	Comparative ∆Ct	RPL18	EIF	β-act	18S	TUB	GAPDH	EF-1α
	BestKeeper	RPL18	EIF	18S	β-act	TUB	GAPDH	EF-1α
	NormFinder	RPL18	EIF	β-act	18S	GAPDH	TUB	EF-1α
	GeNorm	EIF/RPL18		18S	β-act	TUB	GAPDH	EF-1α
	Recommended comprehensive ranking	RPL18	EIF	18S	β-act	TUB	GAPDH	EF-1α
Different ovarian stages	Comparative ∆Ct	β-act	EIF	RPL18	18S	EF-1α	TUB	GAPDH
	BestKeeper	EIF	EF-1α	β-act	18S	TUB	RPL18	GAPDH
	NormFinder	EIF	β-act	EF-1α	RPL18	18S	TUB	GAPDH
	GeNorm	18S/RPL18		β-act	EIF	EF-1α	TUB	GAPDH
	Recommended comprehensive ranking	EIF	β-act	RPL18	18S	EF-1α	TUB	GAPDH
Different embryo stages	Comparative ∆Ct	EIF	β-act	TUB	18S	GAPDH	RPL18	EF-1α
	BestKeeper	18S	EIF	β-act	TUB	RPL18	GAPDH	EF-1α
	NormFinder	EIF	β-act	GAPDH	TUB	18S	RPL18	EF-1α
	GeNorm	18S/TUB		EIF	β-act	GAPDH	RPL18	EF-1α
	Recommended comprehensive ranking	EIF	18S	β-act	TUB	GAPDH	RPL18	EF-1α
Hypoxia stress	Comparative ∆Ct	β-act	GAPDH	EIF	18S	EF-1α	TUB	RPL18
	BestKeeper	EIF	TUB	GAPDH	β-act	18S	RPL18	EF-1α
	NormFinder	β-act	GAPDH	EIF	18S	TUB	EF-1α	RPL18
	GeNorm	GAPDH / β-act		EIF	18S	EF-1α	TUB	RPL18
	Recommended comprehensive ranking	β-act	GAPDH	EIF	18S	TUB	EF-1α	RPL18
WSSV infection	Comparative ∆Ct	EIF	RPL18	GAPDH	TUB	EF-1α	β-act	18S
	BestKeeper	RPL18	EIF	TUB	GAPDH	EF-1α	18S	β-act
	NormFinder	RPL18	EIF	GAPDH	TUB	β-act	EF-1α	18S
	GeNorm	EIF /TUB		GAPDH	RPL18	EF-1α	β-act	18S
	Recommended comprehensive ranking	EIF	RPL18	TUB	GAPDH	EF-1α	β-act	18S