Table A3.

Reference gene expression stability values based on several programs.

	Rank	∆Ct		BestKeeper		NormFinder		GeNorm
		Genes	Average of Std dev	Genes	Std dev	Genes	Stability Index	Genes	Stability Value
Different adult tissues	1	RPL18	2.22	RPL18	0.83	RPL18	0.252	EIF/RPL18	0.392
	2	EIF	2.24	EIF	0.94	EIF	0.542
	3	β-act	2.52	18S	1.10	β-act	1.767	18S	1.354
	4	18S	2.66	β-act	1.38	18S	1.934	β-act	1.55
	5	TUB	2.91	TUB	1.93	GAPDH	2.128	TUB	1.705
	6	GAPDH	3.07	GAPDH	2.16	TUB	2.369	GAPDH	2.192
	7	EF-1α	4.65	EF-1α	3.74	EF-1α	4.462	EF-1α	2.895
Different ovarian stages	1	β-act	0.85	EIF	0.87	EIF	0.439	18S/RPL18	0.319
	2	EIF	0.88	EF-1α	1.11	β-act	0.506
	3	RPL18	0.91	β-act	1.25	EF-1α	0.609	β-act	0.388
	4	18S	0.95	18S	1.3	RPL18	0.637	EIF	0.513
	5	EF-1α	0.99	TUB	1.38	18S	0.749	EF-1α	0.653
	6	TUB	1.2	RPL18	1.5	TUB	1.032	TUB	0.892
	7	GAPDH	1.33	GAPDH	1.57	GAPDH	1.218	GAPDH	1.016
Different embryo stages	1	EIF	2.37	18S	1.89	EIF	0.642	18S/TUB	1.165
	2	β-act	2.47	EIF	1.73	β-act	0.786
	3	TUB	2.74	β-act	5.07	GAPDH	1.775	EIF	1.44
	4	18S	2.77	TUB	3.11	TUB	1.906	β-act	1.578
	5	GAPDH	2.84	RPL18	2.55	18S	2.059	GAPDH	1.724
	6	RPL18	3.36	GAPDH	2.3	RPL18	2.295	RPL18	2.24
	7	EF-1α	5.35	EF-1α	2.92	EF-1α	5.137	EF-1α	3.129
Hypoxia stress	1	β-act	0.6	EIF	0.49	β-act	0.092	GAPDH/β-act	0.185
	2	GAPDH	0.66	TUB	0.57	GAPDH	0.316
	3	EIF	0.7	GAPDH	0.61	EIF	0.323	EIF	0.434
	4	18S	0.74	β-act	0.67	18S	0.45	18S	0.532
	5	EF-1α	0.95	18S	0.85	TUB	0.822	EF-1α	0.631
	6	TUB	0.97	RPL18	0.93	EF-1α	0.828	TUB	0.72
	7	RPL18	1.01	EF-1α	1.06	RPL18	0.912	RPL18	0.803
WSSV infection	1	EIF	0.66	RPL18	0.31	RPL18	0.297	EIF/TUB	0.397
	2	RPL18	0.67	EIF	0.32	EIF	0.334
	3	GAPDH	0.69	TUB	0.38	GAPDH	0.391	GAPDH	0.454
	4	TUB	0.72	GAPDH	0.41	TUB	0.424	RPL18	0.489
	5	EF-1α	0.91	EF-1α	0.46	β-act	0.737	EF-1α	0.593
	6	β-act	0.92	18S	0.6	EF-1α	0.748	β-act	0.703
	7	18S	1.06	β-act	0.68	18S	0.929	18S	0.805