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. 2018 Aug 2;19(8):2269. doi: 10.3390/ijms19082269

Figure 4.

Figure 4

Effect of AgNPs on reactive oxygen species (ROS) generation. (A) HCT116 cells were treated with or without AgNPs for 24 h, and ROS generation was measured using 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). (B) HCT116 cells were treated with AgNPs for 24 h. After incubation, the cells were harvested and washed twice with ice-cold phosphate-buffered saline (PBS) solution. The cells were collected, and disrupted by ultrasonication for 5 min on ice. Lipid peroxidation (LPO) was determined via the reaction of malondialdehyde (MDA) with thiobarbituric acid to form a colorimetric (532 nm)/fluorometric (excitation and emission wavelengths of 532 and 553 nm, respectively) product, whose quantity was proportional to that of MDA. The results are expressed as the mean ± standard deviation of three independent experiments. There was a significant difference in the ratio for AgNP-treated cells compared to untreated cells, determined by a Student’s t-test (* p < 0.05).