Figure 7.

Impact of AgNPs on gene expression. (A) Scatter plot showing up- and downregulated genes in AgNP-treated HCT116 cells. The log₂ fragments per kilobase of transcript per million mapped reads (FPKM) values were used for plotting. Red and blue dots indicate downregulated and upregulated genes in AgNP-treated HCT116 cells, respectively. Cut-off: FPKM ˃2 and fold change ˃2. (B) Representative IGV images of down- (CCNB1 and CCNB2) and upregulated (CYP1A1 and CYP1B1) genes. (C) Gene Ontology (GO) term analysis using both up- and downregulated genes. Each red (downregulated) or blue (upregulated) dot represents one gene belonging to each term. (D,E) bar graphs showing GO terms of individual up- and downregulated genes.