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. 2018 Aug 17;19(8):2436. doi: 10.3390/ijms19082436

Figure 6.

Figure 6

HDAC3 inhibition resulted in Ucp1 upregulation in IWAT cell. Hdac3 and Ucp1 expression after transfection with Hdac3 siRNA for 24 h without (a) or with (b) 10 µM iso induction for 2 h. (c) HDAC activity after treatment with HDAC3 inhibitor RGFP966 (RGFP) for 24 h. (d) H3K27ac level after induction by 10 µM iso for 4 h, 5 μM RGFP for 24 h, or both compounds. Protein band were quantified by ImageJ. Browning-related gene expression after treatment with 5 μM RGFP for 24 h without (e) or with (f) 10 µM iso induction in the last 4 h. Data are presented as mean ± S.E.M. (error bars). n = 3–6 in each group. *, ** indicate significant differences at p < 0.05 and p < 0.01, respectively, according to unpaired-t test. N.S., not significant.