Table 1.
Differential proteins identified by MALDI-TOF/TOF-MS of why1/3-var and CIPK14-var.
| Spot No a | Protein Name | Accession No b | Mascot Score | Matched Peptides | Theor MW (kDa)/pI c | Cov% d | Subcellular Loc e |
|---|---|---|---|---|---|---|---|
| Light reaction | |||||||
| 1 | Fe-S cluster assembly factor HCF101 | HF101_ARATH | 371 | 13 | 57.728/5.91 | 20% | plastid |
| 2 | RCA | F4IVZ7_ARATH | 499 | 18 | 48.469/7.55 | 34% | chloroplast |
| 3 | ATP synthase subunit alpha | ATPA_ARATH | 927 | 32 | 55.294/5.19 | 50% | plastid |
| 4 | Magnesium-chelatase subunit ChlI-2 | CHLI2_ARATH | 365 | 23 | 46.069/5.36 | 44% | chloroplast |
| 12 | Chlorophyll a-b binding protein CP26 | CB5_ARATH | 213 | 12 | 30.183/6 | 35% | plastid |
| 26 | Magnesium protoporphyrin IX methyltransferase | CHLM_ARATH | 653 | 23 | 33.775/7.68 | 53% | chloroplast |
| 28 | PsbP domain-containing protein 4 | PPD4_ARATH | 122 | 8 | 28.484/7.02 | 33% | plastid |
| 33 | Oxygen-evolving enhancer protein 3-2 | PSBQ2_ARATH | 464 | 16 | 24.628/9.72 | 59% | plastid |
| 34 | PSBR | A0A178WGP6_ARATH | 112 | 7 | 9.77/10.1 | 39% | plastid |
| 35 | TROL | A0A178V0X3_ARATH | 444 | 21 | 54.448/5.09 | 29% | chloroplast |
| Calvin cycle | |||||||
| 5 | Phosphoglycerate kinase 1 | PGKH1_ARATH | 642 | 24 | 50.081/5.91 | 37% | chloroplast |
| 6 | Sedoheptulose-1,7-bisphosphatase | S17P_ARATH | 354 | 18 | 42.388/6.17 | 28% | chloroplast |
| 14 | Ribulose bisphosphate carboxylase large chain (Fragment) | A0A142I795_ARATH | 292 | 12 | 51.833/6.17 | 9% | chloroplast |
| 17 | Ribulose bisphosphate carboxylase small chain | A0A178UL15_ARATH | 326 | 14 | 20.33/7.59 | 45% | chloroplast |
| 18 | Ribulose bisphosphate carboxylase small chain 1A | RBS1A_ARATH | 369 | 15 | 20.203/7.59 | 45% | chloroplast |
| 23 | Ribulose bisphosphate carboxylase large chain (Fragment) | A0A142I795_ARATH | 292 | 12 | 51.833/6.17 | 18% | plastid |
| 27 | Ribulose bisphosphate carboxylase large chain | RBL_ARATH | 740 | 31 | 52.922/5.88 | 42% | chloroplast |
| 32 | Ribulose bisphosphate carboxylase small chain | A0A178UL15_ARATH | 326 | 14 | 20.33/7.59 | 45% | chloroplast |
| 40 | Ribulose bisphosphate carboxylase large chain (Fragment) | A0A142I795_ARATH | 292 | 12 | 51.833/6.17 | 26% | chloroplast |
| 43 | Ribulose bisphosphate carboxylase small chain 1A | RBS1A_ARATH | 362 | 13 | 20.203/7.79 | 49% | chloroplast |
| 45 | Ribulose bisphosphate carboxylase small chain 1B | RBS1B_ARATH | 348 | 14 | 18.506/8.22 | 53% | chloroplast |
| 59 | Beta carbonic anhydrase 1 | BCA1_ARATH | 307 | 15 | 37.426/5.74 | 48% | plastid/ cytomembrane |
| Defense and antioxidation | |||||||
| 7 | Glucan endo-1,3-beta-glucosidase, acidic isoform | E13A_ARATH | 568 | 16 | 37.316/4.85 | 33% | secretion |
| 9 | Thiamine thiazole synthase | THI4_ARATH | 183 | 10 | 36.641/5.82 | 28% | plastid |
| 10 | SAPX | A0A178V0Q5_ARATH | 524 | 24 | 40.446/8.31 | 47% | chloroplast |
| 16 | Glycine-rich RNA-binding protein 7 | RBG7_ARATH | 167 | 9 | 16.88/5.85 | 51% | cytoplasm/nucleus |
| 20 | Glutathione S-transferase F6 | GSTF6_ARATH | 355 | 15 | 23.471/5.8 | 49% | cytoplasm |
| 24 | V-type proton ATPase subunit E1 | VATE1_ARATH | 328 | 30 | 26.044/6.04 | 75% | vacuole |
| 25 | l-ascorbate peroxidase 1 | F4HU93_ARATH | 389 | 16 | 27.503/5.85 | 52% | cytoplasm |
| 37 | Pyridoxal 5′-phosphate synthase subunit PDX1.1 | PDX11_ARATH | 423 | 23 | 32.841/5.75 | 37% | cytoplasm |
| 38 | Thioredoxin-like protein CDSP32 | CDSP_ARATH | 371 | 20 | 33.663/8.65 | 35% | chloroplast |
| 44 | GRP7 | A0A178VQY8_ARATH | 315 | 9 | 16.937/5.85 | 37% | cytoplasm/nucleus |
| 46 | V-type proton ATPase subunit G1 | VATG1_ARATH | 304 | 10 | 12.389/5.77 | 70% | vacuole |
| 51 | Formate dehydrogenase | FDH_ARATH | 354 | 18 | 42.383/7.12 | 36% | chloroplast/mitochondria |
| 52 | 12-oxophytodienoate reductase 3 | OPR3_ARATH | 782 | 24 | 42.664/7.71 | 54% | peroxysome |
| 54 | Thylakoid lumenal 29 kDa protein | TL29_ARATH | 475 | 24 | 37.911/8.59 | 53% | plastid |
| 55 | Remorin | REMO_ARATH | 427 | 26 | 20.955/8.63 | 65% | plasmalemma |
| 57 | VIPP1 | A0A178W0D3_ARATH | 412 | 23 | 28.895/5.9 | 67% | plastid |
| 60 | Glutathione S-transferase F7 | GSTF7_ARATH | 552 | 18 | 23.583/6.14 | 52% | cytoplasm |
| 61 | Peptide methionine sulfoxide reductase A4 | MSRA4_ARATH | 236 | 12 | 38.626/8.96 | 26% | plastid |
| Amino acid metabolism | |||||||
| 22 | Serine hydroxymethyltransferase 4 | GLYC4_ARATH | 504 | 22 | 51.685/6.8 | 46% | cytoplasm |
| 48 | Asparagine synthetase [glutamine-hydrolyzing] 2 | ASNS2_ARATH | 235 | 18 | 64.989/6.01 | 27% | cytoplasm/plasmodesmata |
| 50 | Glutamate-glyoxylate aminotransferase 1 | GGT1_ARATH | 478 | 20 | 53.267/6.49 | 37% | peroxysome |
| 58 | Acetylglutamate kinase | NAGK_ARATH | 424 | 14 | 36.572/9.04 | 36% | plastid |
| Proteometabolism | |||||||
| 11 | ATP-dependent Clp protease proteolytic subunit-related protein 3 | CLPR3_ARATH | 597 | 23 | 36.284/8.64 | 41% | chloroplast |
| 13 | Proteasome subunit alpha type-6-A | PSA6A_ARATH | 926 | 25 | 27.277/5.6 | 58% | cytoplasm/nucleus |
| 15 | 30S ribosomal protein S6 alpha | RR6_ARATH | 119 | 8 | 22.746/5.92 | 26% | plastid |
| 19 | CPN10 | O80504_ARATH | 567 | 10 | 15.04/8.75 | 45% | chloroplast |
| 29 | Proteasome subunit beta type-2-B | PSB2B_ARATH | 715 | 22 | 21.97/6.21 | 70% | cytoplasm/nucleus |
| 30 | Ribosome-recycling factor | RRFC_ARATH | 497 | 18 | 30.403/9.46 | 44% | plastid |
| 31 | Peptidyl-prolyl cis-trans isomerase CYP18-3 | CP18C_ARATH | 307 | 11 | 18.361/7.68 | 34% | cytoplasm |
| 39 | Proteasome subunit alpha type-6-B | PSA6B_ARATH | 312 | 19 | 27.333/5.75 | 56% | cytoplasm/nucleus |
| 56 | Peptidyl-prolyl cis-trans isomerase CYP19-1 | CP19A_ARATH | 406 | 14 | 18.48/8.65 | 43% | cytoplasm |
| 62 | Nascent polypeptide-associated complex subunit beta | A0A178W6R8_ARATH | 332 | 14 | 16.935/5.50 | 45% | cytoplasm/nucleus |
| Energy metabolism | |||||||
| 21 | Beta-amylase 3 | BAM3_ARATH | 510 | 24 | 61.314/6.59 | 41% | chloroplast |
| Malate biosynthesis | |||||||
| 36 | Malate dehydrogenase | MDHP_ARATH | 334 | 12 | 42.379/8.66 | 23% | chloroplast |
| 47 | NADP-dependent malic enzyme 2 | MAOP2_ARATH | 323 | 19 | 64.372/6.01 | 25% | cytoplasm |
| 49 | l-galactono-1,4-lactone dehydrogenase | GLDH_ARATH | 759 | 35 | 68.513/8.7 | 38% | mitochondria |
| Lipid metabolism | |||||||
| 53 | GDSL esterase/lipase At1g29670 | GDL15_ARATH | 841 | 19 | 39.847/8.85 | 44% | secretion |
| Transcription | |||||||
| 8 | RNA-binding protein CP29B | CP29B_ARATH | 602 | 16 | 30.699/5.06 | 52% | plastid |
| Unknow function | |||||||
| 41 | At1g13930/F16A14.27 | Q9XI93_ARATH | 310 | 14 | 16.154/4.82 | 87% | chloroplast/plasmalemma |
| 42 | Kinesin-like calmodulin-binding protein | KCBP_ARATH | 54 | 35 | 143.359/6.69 | 21% | cytoplasm |
a Numbering corresponds to 2-DE gel in Figure 1A–C. b Database accession of the identified proteins in uniprot (http://www.uniprot.org/). c Molecular mass and pI theoretical. d Percentage of predicted protein sequence with match sequence. e Subcellular localization of the identified protein base on uniprot and previous literature.