Table 1.
Summary of significant (three-way align rank transform linear mixed models; p < 0.05) single factor effects and factor interaction effects on cultured human keratinocytes’ proliferative potential. Mean values are displayed.
| 1 Significant Factor Effects and Interactions | Keratinocytes’ Proliferative Potential Proxies | ||
|---|---|---|---|
| Daily Population Doublings | Population Mean Cell Size (μm) | Colony-Forming Efficiency (% of Holoclones) | |
| Feeder layer type | iHFL > i3T3FL | iHFL > i3T3FL | iHFL > i3T3FL |
| 0.93 > 0.66 | 16.32 > 15.85 | 5.85 > 1.81 | |
| cAMP inducer type | cISO > CT | ||
| 4.56 > 3.07 | |||
| Passage number | (P1 = P2) > P3 | P1 < (P2 = P3) | P1 > P2 > P3 |
| (0.89 = 0.83) > 0.66 | 15.56 < (16.20 = 16.50) | 5.58 > 4.01 > 2.00 | |
| 2 Feeder layer type × passage number | iHFL − i3T3FL: P1 < (P2 = P3) | iHFL − i3T3FL: P1 > (P2 = P3) | |
| 0.11 < (0.31 = 0.38) | 1.47 > (0.07 = −0.11) | ||
1 Only significant effects (p < 0.05) are reported in this table. 2 Passage-specific differences of the proxy means for keratinocytes cultured on iHFL when compared to keratinocytes cultured on i3T3FL.