Figure 1.

Y-box binding protein-1 (YB-1) is not translocated to the nucleus after irradiation. (A) A KRASmut breast cancer cell line (MDA-MB-231), non-small cell lung cancer cell lines (A549, H460), and (B,C) KRASwt breast cancer cell lines (HBL-100, MCF7, SKBR3) were irradiated (4 Gy). Cytoplasmic and nuclear protein fractions were isolated at the indicated time points after irradiation and subjected to SDS-PAGE. YB-1 was detected by Western blotting. α-Tubulin and lamin A/C were used as cytoplasmic and nuclear markers, respectively. (D) Histogram representing the densitometry data for the mean expression of YB-1 ± S.E.M. within one hour after ionizing radiation (IR) from four independent experiments in HBL-100 cells.