Table 3.
Online transcriptomics resources in wheat.
| Resources | Description/URL |
|---|---|
| Genome sequence | Coordinated effort underway by the IWGSC (http://www.wheatgenome.org) Recognized as a priority by the research community (http://www.csrees.usda.gov/nea/plants/pdfs/wheat_conference_summary.pdf) |
| ESTs 1 | 1,050,791 entries |
| Oligonucleotide microarray | 1,050,791 entries |
| cDNA microarray | Multiple including ~9 K array |
| Tiling microarray | Not currently available |
| Serial Analysis of Gene Expression (SAGE) | Applied for studying, developing wheat caryopsis |
| Massively Parallel Signature Sequencing (MPSS) | Not reported |
| Sequencing-by-synthesis | Roche 454 cDNA sequencing [119] |
| Deletion and aneuploid genetic stocks | Roche 454 cDNA sequencing [119] |
| Transformation | Biolistic- and Agrobacterium-mediated DNA delivery systems |
| Gene knockdown | RNA interference Viral-induced gene silencing |
| Databases/tools | Graingenes (http://wheat.pw.usda.gov) Gramene (http://www.gramene.org) TIGR Genome Database (http://www.tigr.org/tdb/e2k1/tae1) Wheat Genome Project (http://wheat.pw.usda.gov/NSF/htmlversion.html) Wheat Genome Project (http://wheat.pw.usda.gov/NSF/htmlversion.html) wEST (http://wheat.pw.usda.gov/wEST) CerealsDB (http://www.cerealsdb.uk.net) HarvEST Wheat (http://harvest.ucr.edu) PLEXdb (http://plexdb.org) Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo) ArrayExpress (http://www.ebi.ac.uk/microarray-as/ae) GrainSAGE (http://www.scu.edu.au/research/cpcg/igfp/index.php) Wheat SNP Project (http://wheat.pw.usda.gov/SNP/new/index.shtml) [120] |
Esterase, 1 ESTs listed in the National Center for Biotechnology Information (NCBI) EST database (GenBank dbEST) (8 August 2008; http:// www.ncbi.nlm.nih.gov/dbEST/dbEST_summary.html).