Figure 1.

Localization of Tspo mRNA in healthy and diseased prostate tissues: In normal tissue (A,B) Tspo mRNA is localized to the cytoplasm of the inner columnar epithelium (C) and in the cytoplasm and elastic fibres (FM) and in the nuclei of fibroblasts of the fibromuscular stroma. Transcription of Tspo increases in hyperplastic tissue, (C,D) as shown by the increase in fluorescence intensity (D). In Grade II adenocarcinoma (E,F) Tspo mRNA is localized within the cytoplasm of cuboidal to low columnar epithelial cells lining glands (G), in the collagen fibres located in the stroma and in the nuclei and cytoplasm of lymphocytes (L) and endothelial cells lining blood vessels located in the surrounding stroma. In Grade III adenocarcinoma (G,H) Tspo mRNA is found in the cytoplasm of tumor cells that grow in sheets. Negative control for the subcellular localization of Tspo mRNA (I,J). Sense probes were used to probe tissue preparations. Tissues are magnified at 1000×.