Figure 6.

Subcellular Localization of Tspo mRNA in healthy and diseased Kidney Tissue: In healthy kidney tissue (A–D). Tspo mRNA is expressed in the cytoplasm of the flattened cells located in the parietal layer of the Bowman’s capsule, in the cytoplasm of endothelial cells that line the anastomosing network of capillaries of in the glomerulus (G), in the cytoplasm of the simple cuboidal epithelium that line the proximal convoluted tubule (PCT), in the cytoplasm of the simple squamous epithelium and erythrocytes within the vasa recta, in the cytoplasm of the simple squamous epithelium that lines the thin ascending and descending limbs (T), in the cytoplasm of the low cuboidal epithelium of the thick ascending limb (A), in the cytoplasm of the simple cuboidal epithelium of the distal convoluted tubule (DCT), in the cytoplasm of the simple cuboidal epithelium of the collecting tubule shape to the ascending limb and in the cytoplasm of the simple columnar epithelium that lines the collecting duct (CD). The transcription level of Tspo mRNA decreases in renal cell carcinoma (F,H) versus healthy kidney tissue (B). In Grade III chromophobe renal cell carcinoma (E,F) the mRNA is localized in the nuclei and cytoplasm of tumour cells that are usually arranged as a solid pattern with concentrations of the largest cells around the blood vessels. In renal clear cell carcinoma (G,H) the mRNA localizes in the nuclei and cytoplasm of tumour cells and in the cytoplasm of the delicate branching vasculature that appears fibromuscular. (I,J) Negative control for the subcellular localization of Tspo mRNA. Sense probes were used to probe tissue preparations and this was analysed colorimetrically and fluorescently. Tissues are magnified at 1000×.