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. 2018 Jul 31;19(8):2230. doi: 10.3390/ijms19082230

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The cellular uptake of the NP/siRNA complexes in U-87 cells. (A) The interaction of the PEI-coated Fe₃O₄ NPs with siRNA at different NP/siRNA weight ratios, analyzed by gel retardation assay; (B) the gray value analysis for the results of gel retardation assay. * p < 0.05 compared with the 0 group; (C) the cellular uptake of the NP/siRNA complexes in U-87 cells was verified by prussian blue staining, fluorescence labeling and flow cytometry, P2 presented positive proportions. Bar indicates 100 μm.