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. 2018 Jul 31;19(8):2230. doi: 10.3390/ijms19082230

Figure 4.

Figure 4

Repressor element 1-silencing transcription factor (REST) silencing mediated by NP/siRNA complexes in GBM cells. (A) The mRNA levels of REST in U-87 and U-251 cells incubated with NP/siRNA complexes (at the ratio of 4 h for 24 h) targeting REST, assayed by real-time polymerase chain reaction (PCR); (B) The protein levels in U-87 cells treated with NP/siRNA complexes targeting REST were detected by Western blotting; (C) The protein levels in U-251 cells treated with NP/siRNA complexes targeting REST were detected by western blotting. * p < 0.05 compared with the control group.