Figure 5.

Prion protein structures and analysis of Asn deamidation. (A) The structure of PrP^C and metal-binding sites. Octarepeat domain and neurotoxic fragments PrP^106–126 are depicted and Asn¹⁰⁸ is indicated by underline. PrP^C possesses six metal-binding sites. The PrP^106–126 was analyzed with a mobile phase containing 20% acetonitrile, a 15 mM sodium phosphate solution (pH 5.0), and 100 mM NaCl according to the methods described in [14]. The HPLC profiles after incubation for 28 days at 37 °C in 50 mM phosphate buffer (pH 7.4) and the summarized graph are shown (Y. Sadakane, unpublished data). (B) Asn deamidation and Asp isomerization in rodent prion protein are shown [87].