Table 2.
Primers used for cloning and analysis of CsPP1 and CsPPO2.
| Primers | Purpose | Primer Sequence (5′-3′) |
|---|---|---|
| GSP1-3 | 3′-RACE | AATGTGGATCGGATGTGG |
| NGSP1-3 | 3′-RACE | CAGAGACCGAAGAAATCAAG |
| GSP2-3 | 3′-RACE | GGTTTGTGTTCTATGATGAG |
| NGSP2-3 | 3′-RACE | AGAAGGATGATGAAGAGGAG |
| GSP1-5 | 5′-RACE | GAGCCATGAGTTGTGGACTTGAAG |
| NGSP1-5 | 5′-RACE | TGGTGATAAGCTCCGTCGCAATAA |
| GSP2-5 | 5′-RACE | ATCCGACCCGTTGTAATCCA |
| NGSP2-5 | 5′-RACE | CGAAGAAGTGGAGGTAGTAT |
| UPM | RACE | Long primer: CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT |
| Short primer: CTAATACGACTCACTATAGGGC | ||
| PPO1-full1 | Clone | ATGAATTCTCTTCCACCATCA |
| PPO1-full2 | Clone | TTAGGAATCAAACTCAATCTTG |
| PPO2-full1 | Clone | ATGGCTTCTTTTCCACCTTC |
| PPO2-full2 | Clone | TCAAGAATCAAACTCTATCTTGA |
| PPO1-PF | protein | CTGGTTCCGCGTGGATCCATGAATTCTCTTCCACCATCATGCA |
| PPO1-PR | protein | CGCTCGAGTCGACCCGGGTTAGGAATCAAACTCAATCTTG |
| PPO2-PF | protein | CTGGTTCCGCGTGGATCCATGGCTTCTTTTCCACCTTC |
| PPO2-PR | protein | CGCTCGAGTCGACCCGGGTCAAGAATCAAACTCTATCTTGA |
| GAPDH-F | QPCR | GACTGGAGAGGTGGAAGAGC |
| GAPDH-R | QPCR | AGCCATTCCAGTCAATTTCC |
| PPO1-RTF | QPCR | CCATCTGGAAGAGTTTGGGT |
| PPO1-RTR | QPCR | CCTTCACTTTGACAGGCTGA |
| PPO2-RTF | QPCR | CGGAATGCCAATGCCTGCAA |
| PPO2-RTR | QPCR | AGTTGGATCCGACCCGTTGT |