. Author manuscript; available in PMC: 2018 Sep 4.

Published in final edited form as: Environ Mol Mutagen. 2017 Apr;58(3):162–171. doi: 10.1002/em.22085

Table I.

Mutagenicity of EOM of Oil-Burn Emissions

	Dose	Rev/plate^a

	(µg EOM/	−S9			+S9

Strain	plate)	Exp 1	Exp 2	Exp 3	Exp 1	Exp 2	Exp 3
TA100	0	127	121		130	120
	1	108			130
	5	112			158
	10	168	149		258	269
	25	195	175		383	416
	50	229	182		599	529
	100	245	264		809^b	707^b
	NaN₃	722	732
	2AA				871	895
	Blank	129			109

YG1041	0	87	89		81	106
	1	77			70
	5	104			107
	10	142	168		147	170
	25	202	265		200	248
	50	380	391		346	377
	100	535	616		643	633
	2NF	1648	1547
	2AA				1237	1786
	Blank	100			118

TA98	0	43	56		49	64
	10	44	60		86	92
	25	70	82		99	102
	50	106	95		161	152
	100	115^b	119^b		256	250
	2NF	372	305
	2AA				297	594
	Blank	38	45		59	75

TA104	0	225	210	199	296	273	279
	10	243			383
	25	278	268	240	453	494	513
	50	295	292	287	535	602	568
	100	322	348	345	588^b	635^b	716^b
	252.5		458	454		906^b	856^b
	MG	1338	1563	1153
	2AA				497	655	565
	Blank	221	253		285	326

YG1024	0	30	33		46	48
	10	99	50		108	94
	25	120	90		222	128
	50	177	132		345	215
	100	199^b	181^b		360^b	323^b
	2NF	2116	2065
	2AA				1769	2226
	Blank	23	33		48	40

YG1042	0	108	108		112	123
	10	138	133		253	215
	25	188	155		362	312
	50	167	181		554	414
	100	232	191		711^b	599^b
	NaN₃	977	956
	2AA				621	830
	Blank	115	109		123	115

Data for DMSO (0) controls and the positive controls are the average ± SE of 3 plates each per experiment for a total of 6 plates. Data for the method blanks are for 100 µl/plate and are the average of 2 plates (1 each from 2 independent experiments) except for TA100 and YG1041, which are based on 1 plate from only 1 experiment. Data for the EOM are single plates/dose for each experiment. The positive control data for each experiment represent the average of 3 plates. The positive controls were 2-nitrofluorene (2NF) at 3 µg/plate, 2-aminoanthracene (2AA) at 0.5 µg/plate, sodium azide (NaN₃) at 3 µg/plate, and methylglyoxal (MG) at 200 µg/plate. The mean ± SE rev/plate of the DMSO controls for all experiments combined for −/+ S9 were 124 ± 3/125 ± 5 for TA100, 88 ± 1/94 ± 13 for YG1041, 50 ± 7/57 ± 8 for TA98, 211 ± 8/283 ± 7 for TA104, 32 ± 2/47 ± 1 for YG1024, and 108 ± 0/118 ± 6 for YG1042. The mean ± SE rev/plate of the method blanks for all experiments combined for −/+ S9 were 129/109 for TA100, 100/118 for YG1041, 42 ± 4/67 ± 8 for TA98, 237 ± 16/306 ± 21 for TA104, 28 ± 5/44 ± 4 for YG1024, and 112 ± 3/119 ± 4 for YG1042. The mean ± SE rev/plate for the positive controls for all experiments combined for −/+ S9 were 727 ± 5 NaN₃/883 ± 12 2AA for TA100, 1598 ± 51 2NF/1512 ± 275 2AA for YG1041, 339 ± 34 2NF/446 ± 149 2AA for TA98, 1155 ± 302 MG/768 ± 198 2AA for TA104, 2091 ± 26 2NF/1998 ± 229 2AA for YG1024, and 967 ± 11 NaN₃/726 ± 105 2AA for YG1042.

These data were not used in the linear regressions because they were outside of the linear portion of the dose-response curves.